UV

UV/VIS Spectrophotometer -Training:

UV/VIS Spectrophotometer is used to determine the absorption or transmission of UV/VIS light by a sample. It can also be used to measure concentrations of absorbing materials based on developed calibration curves of the material. A sample is placed in the UV/VIS beam and a graph of the transmittance or absorbance versus the wavelength is obtained.

Alternatively, samples are prepared in known concentrations and their absorbance is read by the UV/VIS Spectrophotometer. Results are then graphed to make a calibration curve from which the unknown concentration can be determined by its absorbance.

A spectrum of percent transmittance or absorbance versus wavelength. The concentration can be found by mathematical methods based on Beer’s Law.

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